NGSI offers an unprecedented protocol for immunostaining. The conventional immunostaining method must require several steps such as blocking and repetitive washing steps. It is time-consuming and labor-intensive. However, NGSI is the one step stamping procedure for antibody labeling within 5 minutes. NGSI also enables multiple immunostaining quickly and easily with continuous stamping while exchanging new gel containing other antibodies. This is to provide a new solution for IHC in cancer diagnosis.